Thursday, August 21, 2014

Applications: Monitoring Calcium Flux in 3D Heart Tissues in Real Time

In order for a drug to attain approval for use it must not only be shown that it has the desired effect but that it does not have undesirable side-effects. Traditionally, laboratory animals are used to predict possible side-effects but the animals may respond differently than humans so an effective in vitro system is highly desirable.

In order for an in vitro system to be most useful it should best mimic the in vivo tissue. For this reason 3D cell culture approaches have become more prevalent and have proven to exhibit fewer misleading results than their 2D counterparts.

The scientists at InvivoSciences have recognized the importance of using 3D cultures for drug assessment and have met the challenge of creating a suitable in vitro system with their Mini-Construct ChambersTM .

The CLARIOstar® captures periodic Ca2+-transients 
that induce cardiac contractions

In an application note now available on the BMG LABTECH website InvivoSciences scientists describe the use of the CLARIOstar® to monitor the function of engineered heart tissues. A calcium indicator was used to monitor the calcium transients that induce cardiac contractions. The ability of the CLARIOstar® to read 100 data points per second allow it to measure the rapid changes in fluorescence intensity that are associated with the calcium flux from a beating heart.

Together, BMG and InvivoSciences can provide a platform for the analysis of drug effects on heart function and do so at the early of stages drug discovery!

BMG LABTECH Application Note 253: Real time calcium flux measurements in iPSC derived 3D heart tissues.