|Protein crystals grown in space:|
A collage of protein crystals many of which
were grown on the U.S. Space Shuttle. All the
crystals are useful for X-ray diffraction analysis
A recent Nature Structural & Molecular Biology paper entitled: 'Molecular basis for N-terminal acetylation by the heterodimeric NatA complex' describes the work which resulted from a colaboration between scientists in the USA and Norway. They employed X-ray crystallography and solved the structure of the entire NatA complex and the structure of the catalytic subunit alone. They found that binding of an auxillary protein induces a conformational change in the catalytic subunit which essentially acts as a switch that turns on the enzymatic activity.
Because of the numerous proteins affected by NatA, and its association with cancer, the authors now seek to identify small molecule inhibitors for NatA. The solved structure will allow them to compare this enzyme to other enzymes with known inhibitors. These inhibitors can then be used as the basis for a directed approach to screen for inhibitory compounds. In order for a compound screen to be performed an enzyme specific assay will need to be designed that can be used in high throughput screening.
In order to perform high through put screening you want a microplate reader that is both sensitive and fast. The PHERAstar FS is our most powerful microplate reader whose specialty is high through put and can save you time and money by reducing reagent costs.
For more information on how these and other microplate readers from BMG LABTECH can help you with assay design and high through put performance please visit our website: www.bmglactech.com