|Structure of RNase H (PDB id: 1JL1) generated |
using PyMol with preset: pretty
The key to the assay was designing fluorometric substrates in which a fluorescein labeled oligonucleotide was annealed to a complementary dabcyl-labeled oligonucleotide which serves as a fluorescence quencher. Substrate degradation leads to release of a fluorescein labeled fragment and consequent increase in fluorescent signal. An RNase H2 selective substrate was designed and inhibitor specificity was confirmed using RNase A and DNase I with their similarly designed selective substrates.
This is just another example of how BMG LABTECH microplate readers are assisting in research around the world!