Monday, May 6, 2013

Did you know: The CLARIOstar can be equipped with an AlphaScreen® Laser

Assay Principle of the AlphaScreen® SureFire® Phospho-ERK1/2 assay
Amplified Luminescent Proximity Homogeneous Assay (Alpha) technology represents a distinct approach to sensing proximity; which can be used to study protein-protein interactions. Unlike FRET or BRET, which use resonant energy transferred from a fluorescent or bioluminescent donor respectively, AlphaScreen® uses diffusion of singlet oxygen from donor to acceptor beads. When donor beads are excited at a wavelength of 680 nm; oxygen is converted to singlet oxygen. If the acceptor bead is in proximity; the singlet oxygen initiates a series of chemical reactions that produce a luminescent signal with a wavelength in the range of 520-620 nm. Since singlet oxygen has a short lifetime the donor and acceptor must be within 200 nm of one another. This technology has been employed in AlphaScreen® and AlphaLisa® applications that are based on the sandwich ELISA approach.

The AlphaScreen® laser that BMG LABTECH employs for excitation was first developed in the PHERAstar. Using a laser is helpful as an intense light source may be required to generate a signal. The same laser has now been implemented in the CLARIOstar. This should open the door to many life science labs to be able to work with this powerful technology.

The AlphaScreen® laser in the CLARIOstar is just another reason why with the CLARIOstar ANYthing is possible.