| Degranulation processes 1 - antigen; 2 - IgE antibody;|
3 - FcεRI receptor; 4 - preformed mediators
(histamine, proteases, chemokines, heparine);
5 - granules; 6 - mast cell;7 - newly formed mediators
(prostaglandins, leukotrienes, thromboxanes, PAF)
Since this procedure typically uses a solid phase to which antigens are bound; several washing and incubation steps are necessary before the final fluorescent signal can be detected. So, in addition to not being colorimetric, the type of mix and read assay depicted in the episode is not entirely accurate. However,this is an application that could certainly employ a PHERAstar FS to perform the final analysis!
We thank CSI: Las Vegas for considering our reader for use on their show. We at BMG LABTECH were happy to help!