Friday, April 26, 2013

Focus on: Dual Luciferase assays

BMG LABTECH recently added an application note to our online database entitled: 'Simultaneous dual-emission detection of luciferase reporter assays'. This note describes the performance of the Thermo Scientific™ Pierce™ Luciferase Dual-Spectral Assays using the POLARstar Omega from BMG LABTECH.

Unlike the traditional dual luciferase assays which measure luciferase sequentially and require quenching of the first signal so that the second signal may be detected, the use of spectrally resolved luciferases allows for simplified one step detection. By pairing red firefly luciferase with green RenillaGaussia or Cypridina luciferase and selecting appropriate filters the activity of both luciferase enzymes can be detected in one sample. As a result you will be able to perform gene expression analysis in a simplified platform which is made even easier using the simultaneous dual emission detection capability of the POLARstar Omega.