|Schematic of a luciferase reporter assay|
Dual luciferase reporters have become the go to assay to assess changes in gene expression that result from altering the activity of components of a biological pathway. The standard approach employs sequential detection of one luciferase that relates to the studied subject and a second luciferase that is used to indicate cell viability and transfection efficiency.
In BMG LABTECH application note 233, we describe the use of the POLARstar Omega to perform the Thermo Scientific Pierce Luciferase Dual-Spectral Assays. Since these assays employ spectrally resolved pairs of luciferase enzymes it allows the user to perform one-step detection of both enzymes. The capability to perform simultaneous dual emission detection allows for one read assessment using the POLARstar Omega.
|Gaussia-Firefly Dual-Spectral |
To find out more about the capabilities of the POLARstar Omega please visit bmglabtech.com.