Thursday, March 14, 2013

Applications Thursday: Real-time Quaking Induced Conversion Assay

RT-QuIC sensitivity: analysis of dilutions of scrapie hamster
brain homegenate stock at indicated concentrations. The 50%
seeding dose was achieved with a 2 ul aliqout (seeding dose)
of a 10E-9 dilution of the stock (dark blue line)
From BMG application note 232
The RT-QuIC assay allows you to perform quantitative analysis of prion seeding activity using the FLUOstar Omega from BMG LABTECH. This approach has been applied to many types of prion assays including sheep scrapie and deer chronic wasting disease.

The recent publication: 'Prion Seeding Activities of Mouse Scrapie Strains with Divergent PrPSc Protease Sensitivities and Amyloid Plaque Content Using RT-QuIC and eQuIC' describes the extension of this application to mouse models. This is important as murine models are central to transmissible spongiform encephalopathy pathogenesis research.

Using this approach the authors were able characterize multiple scrapie strains and predict that there will be multiple interesting applications of this approach for mouse adapted strains.

For more information on this application please read application note 232 available on the BMG LABTECH website.