Thursday, February 28, 2013

Applications Thursday: Multiple applications, one reader

A recent article published by Ndjomou et al in the journal Antiviral Research highlights the ability of the FLUOstar Omega to perform a variety of applications. The paper entitled: 'Fluorescent Primuline Derivatives Inhibit Hepatitis C Virus NS3-Catalyzed RNA Unwinding, Peptide Hydrolysis and Viral Replicase Formation' used the microplate reader from BMG LABTECH in four separate applications.

WHO-Guide: Hepatitis C, 2002
The effect of the compounds being studied on HCV replication was assessed using an HCV Renilla luciferase reporter gene assay that employed the optional on board injectors available on the FLUOstar Omega and the luminescent detection capabilities. Luminescent detection was also used in a cell viability assay to assess compound cytotoxicity.

The fluorescence detection capabilities of the FLUOstar Omega were used in an NS3 protease activity assay that employed a 5-Carboxyfluorescein-labeled substrate. Fluorescence detection was also employed in a  molecular beacon-based helicase assay (MBHA) in which DNA or RNA test strands were labeled with fluorophores and/or quenchers which are initially annealed to a complimentay strand. The released strands were designed to form hairpins to prevent reannealing. Therefore helicase activity could be determined based on a change in fluorescence intensity.

In the end 4 distinct tests, using 2 detection modes on 1 multi-functional microplate reader from BMG LABTECH.