Friday, January 25, 2013

Focus on: PHERAstar based primary screens at Sanford-Burnham

The PHERAstar FS  from BMG LABTECH is a highly versatile instrument. This is evident from the variety of applications that the Burnham Center for Chemical Genomics has employed in the design of primary screens using the PHERAstar FS. They have studied post-translational modification, mitochondrial permeability, protease activity and protein-protein interactions and have performed these screens using TR-FRET, fluorescence intensity, absorbancefluorescence polarizationHTRF or FRET detection all on one microplate reader!
The PHERAstar FS from BMG LABTECH

For more information on the primary screening assays please use the following links to the appropriate pages at PubChem:

uHTS identification of inhibitors of cullin neddylation in a TR-FRET assay [Primary Screening]

Single concentration confirmation of uHTS inhibitor hits of the mitochondrial permeability transition pore via a fluorescent based assay [Primary Screening]

Single concentration confirmation of uHTS inhibitor hits of Sentrin-Specific Protease 8 using Nedd8 Protein Substrate [Primary Screening]

uHTS identification of small molecule inhibitors of the mitochondrial permeability transition pore via an absorbance assay [Primary Screening]

uHTS identification of modulators of interaction between CendR and NRP-1 using Fluorescence Polarization assay [Primary Screening]

Single concentration validation of uHTS RPN11 inhibitor hits using a MMP-2 Fluorescence assay [Primary Screening]

Single concentration confirmation of uHTS inhibitor hits from RPN11 in a Fluorescence Polarization assay [Primary Screening]

uHTS HTRF interference counterscreen assay for validation of inhibitors of SUMOylation [Primary Screening]

Identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. [Primary Screening]

uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb via a fluorescence resonance energy transfer (FRET) assay. [Primary Screening]

Single concentration confirmation of uHTS for the identification of UBC13 Polyubiquitin Inhibitors via a TR-FRET Assay [Primary Screening]

uHTS identification of UBC13 Polyubiquitin Inhibitors via a TR-FRET Assay [Primary Screening]