|WHO-Guide: Hepatitis C, 2002|
FLUOstar Omega were used in an NS3 protease activity assay that employed a 5-Carboxyfluorescein-labeled substrate. Fluorescence detection was also employed in a molecular beacon-based helicase assay (MBHA) in which DNA or RNA test strands were labeled with fluorophores and/or quenchers which are initially annealed to a complimentay strand. The released strands were designed to form hairpins to prevent reannealing. Therefore helicase activity could be determined based on a change in fluorescence intensity.
In the end 4 distinct tests, using 2 detection modes on 1 multi-functional microplate reader from BMG LABTECH.