Thursday, December 20, 2012

Applications Thursday

Researchers at Rocky Mountain Laboratories in Hamilton, MT with collaborating scientists in Japan have developed a new assay for quantitating prions for neurodegenerative disease research in transmissible spongiform encephalopathies like Scrapie and Creutzfeldt-Jakob Disease. This new assay, Real-Time Quaking Induced Conversion Assay (RT-QuIC) is faster, of a higher throughput, and more sensitive as compared to previous test methods using biological tissue samples from infected animals. This assay can be measured using BMG LABTECH’s Omega series of  microplate readers. With the Omega’s powerful control and analysis software, researchers can easily perform this assay in several hours to a few days.

For more information please see their publication Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays in PLoS Pathog 6(12): e1001217. Further information can also be found on our customer focus page and on an application note  detailing how to run the assay on an Omega microplate reader.

Figure legend: Magnified 100X, and stained with H&E (hematoxylin and eosin) staining technique, this light photomicrograph of brain tissue reveals the presence of prominent spongiotic changes in the cortex, and loss of neurons in a case of variant Creutzfeldt-Jakob disease (vCJD). This image is courtesy of CDC/Teresa Hammett. Photo Credit: Sherif Zaki; MD; PhD; Wun-Ju Shieh; MD; PhD; MPH