Friday, December 7, 2012

Focus On: Dual Emission Detection for FP, FRET, TR-FRET, BRET, and Dual Glow Assays

Dual Emission Detection is a feature in BMG LABTECH microplate readers that allows them to measure two emission wavelengths with only one read of the microplate. This greatly benefits assays like Fluorescence Polarization (FP), Forster resonance energy transfer (FRET), Time-Resolved-FRET (TR-FRET), Bioluminescence resonance energy transfer (BRET), and Dual Glow assays

The benefits include faster measurement times (twice as fast) because it only has to read the plate once. But more importantly it eliminates read-to-read variations that can occur, such as different well volumes, concentrations, meniscus effects, or fluctuations in excitation energy. The result is an assay that is faster, is more sensitive, has a higher Z' factor, and has a lower %CVs.

Dual Emission Detection is found on the PHERAstar series of HTS microplate readers, as well as on the POLARstar Omega and POLARstar OPTIMA.

Dual Emission in the PHERAstar