Readers like BMG LABTECH’s PHERAstar FS can focus precisely from the bottom of the well and measure cell-based assays in scanning mode, taking hundreds of data points per well to create a fluorescence intensity map. It isn’t possible to see what’s happening in individual cells, but it’s easy to see cell coverage and uniformity, or where in the well expression is happening. An assay that can take two hours on an HCS system would take only eight minutes on the PHERAstar FS, meaning that researchers can collect more data per hour and that expensive HCS equipment is free to be used for methods that truly require its capabilities.
|This figure shows the well scanning capabilities of the PHERAstar FS |
using a 384-well plate seeded with cells expressing different levels of GFP.
HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway.
For more information on Developing and Optimizing Cell-based Microplate Assays, join us for our webinar on Wednesday, November 28. More information can be found here: