Tuesday, November 6, 2012

Did you know that you no longer need injectors to perform dual reporter assays using luciferase?

Photinus pyralis Firefly glowingStable and bright luminescence reporters like red-luciferase, green-Renilla, Gaussia and Cypridina lets users measure two luciferase constructs at once using a long-lived luminescence suitable for liquid handling. Traditional dual reporter assays, like dual luciferase, use time and two different injections to allow users to discriminate between both reporters. Glow-type dual reporter assays use two luciferase constructs that each emit a light at a different wavelength, meaning that a luminometer capable of accepting filters, like any of BMG LABTECH’s Omega microplate readers, can discriminate between two different luciferase enzymes emitting at the same time.

For more information about some of these newer luminescence assays please see Pierce or Promega.