Friday, September 28, 2012

Focus On a Fluorescent Alternative Assay to the Caco-2 Study for Drug Membrane Permeability

New technologies and methods are continuously created that supplant outdated methods in order to save time, money, and to be safer. Now with the PHERAstar FS microplate reader, there are safer, faster, and more cost-effective alternatives to some popular radioactive assays.

One such assay is the Fluorosome trans-PGP application that can replace the Caco-2 assay. This assay measures membrane permeability through the action of P-glycoprotein (PGP) pump. Using purified PGP protein in liposomes, this assay occurs in minutes rather than days and is specific for just the PGP protein.

For more information see the applications noteA faster, more reliable alternative is the Fluorosome®-trans-PGP assay from the Fluorosome Company